Polymerase chain reaction in exploring endodontic infections
نویسندگان
چکیده
Tradit ional ly cult ivat ion, microscopic and immunological based techniques were only the means by which endodontic microbiota were studied. Because of their several limitations in microbiological diagnosis, newer molecular genetic methods were introduced. Polymerase chain reaction -PCR is a widely spread advance in clinical diagnostic technology and known to be cornerstone in genome sequencing projects. Fortunately, PCR has been the focus of a great deal of research in recent years with the goal of exploring microbiota. Here we review the priciples of PCR techniques, PCR derivatives and applications of Real-Time PCR in dental sciences. Data sources: Studies published in the medical, dental and biological literature. Conclusion: PCR has enabled the detection of bacterial strains which are phenotypically divergent or convergent in behaviour. PCR demonstrated a great range of diversity in endodontic microbiota.
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